The development of gene targeting and animal cloning thechnology makes the site - specific integration of foreign gene become possible 隨著基因打靶技術(shù)和動物克隆技術(shù)技術(shù)的發(fā)展,外源基因在高等哺乳動物基因組中的定點(diǎn)整合成為了可能。
To determine the site - specific integration of the foreign gene into the chloroplast genome and the level of homogeneity , the three transformation lines of the second selective generation were analysized through pcr ( pcr - southern blot ) analysis using the primers of fmdv vp1 gene and homologous fragment of tobacco chloroplast , respectively 經(jīng)過兩輪抗生素篩選后,采用fmdvvp1基因的一對引物和煙草葉綠體同源片段的一對引物分別對抗性植株的葉綠體dna進(jìn)行的pcr和pcr - southernblot分析。